GABA-A R alpha 2 Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS024

R&D Systems, part of Bio-Techne
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PPS024
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Primate

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

View all GABA-A R alpha 2 Antibodies »

Product Specifications

Immunogen

GABAA R alpha2 subunit specific peptide

Specificity

Human, mouse, rat and bovine ~53 kDa GABAA R alpha2 subunit

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Scientific Data Images for GABA-A R alpha 2 Antibody

Detection of GABAAR a2 antibody by Western Blot.

Detection of GABAAR alpha2 by Western Blot.

Western blot of 10 μg of rat cortex lysates showing immunolabeling of the ~53 kDa alpha2 subunit of the GABAAR.

Applications for GABA-A R alpha 2 Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain (cortex) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50 μL unpurified neat rabbit serum.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 2

GABAA ( gamma-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, delta, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat alpha2 isoform is a 53 kDa, 423 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is likely to be used as a regulatory mechanism. The alpha2 subunit is known to be phosphorylated on Ser/Thr residues. Rat alpha2 has multiple isoforms, all involving the 5′ UTR. These may dictate alpha2 levels within the cell. This receptor subunit is particularly abundant in forebrain areas and is a potential target of anabolic steroids. Behavioral alterations associated with steroid abuse may be a manifestation of alpha2 beta3 gamma2L receptor activation.

References

  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Khrestchatisky, M. et al. (1991) J. Neurochem. 56:1717.
  4. Yang, P. et al. (2005) Neuropharmacology 49:300.
  5. Tian, H. et al. (2005) Mol. Brain Res. 137:174.
  6. Bureau, M.H. and J.J. Laschet (1995) J.Biol. Chem. 270:26482.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-2 Polypeptide

Alternate Names

GABAAR alpha 2, GABAARa2, GABRA2

Entrez Gene IDs

2555 (Human); 14395 (Mouse); 289606 (Rat); 282236 (Bovine)

Gene Symbol

GABRA2

Additional GABA-A R alpha 2 Products

Product Documents for GABA-A R alpha 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GABA-A R alpha 2 Antibody

For research use only

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