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GABA-A R beta 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-199

Novus Biologicals, part of Bio-Techne
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NB300-199
NB300-199-0.02ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Fusion protein from the cytoplasmic loop of the beta 3 subunit. Accession # P63079

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 21901840).

Specificity

Specific for the ~53 kDa beta 3-subunit of the GABAA receptor in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-A R beta 3 Antibody

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Immunostaining of mouse retina showing specific labeling of the GABAA beta3 subunit in green, calbindin in red and DNA in blue.
Western Blot: GABA-A R beta 3 Antibody [NB300-199]

Western Blot: GABA-A R beta 3 Antibody [NB300-199]

Western Blot: GABA-A R beta 3 Antibody [NB300-199] - Western blot of whole-olfactory bulb homogenates using the B3-subunit antibody in rAAV-Cre-injected versus non-injected GABRB3loxP/loxP mice (each six samples from three mice). The B3-subunit appears as a band of 55 kDa, consistent with its predicted molecular weight. Densitometric analysis (mean+/-s.e.m.) revealed a significant difference (t-test; *P=0.016). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms9950) licensed under a CC-BY license.
Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Each channel was filtered frame by frame. The raw data was acquired at high confocal resolution (XY: 234.32 um x 234.32 um, pixel size = 0.114 um) using a 63x glycerol objective (NA=1.3). Z-steps were 0.15 um per frame. Acquired raw data (1) was filtered using an anisotropic diffusion 2D filter (2). (5). The final images (6) were obtained by thresholding the immunosignal. Scale bars: 10um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms9950) licensed under a CC-BY license.

Applications for GABA-A R beta 3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:300

Simple Western

reported by internal validation

Western Blot

1:1000
Application Notes
Use in Immunohistochemistry free-floating reported in scientific literature (PMID: 26592770). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 17021187). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23430456). In Simple Western internal validation: Human Brain lysate at 0.5 mg/ml as sample; separated by size; antibody dilution of 1:100; observed molecular weight was 48 kDa;matrix was 12-230 kDa; detected by Chemiluminescence.

Reviewed Applications

Read 2 reviews rated 4.5 using NB300-199 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/ml BSA, 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA-A R beta 3

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Long Name

gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide

Alternate Names

GABAAR beta 3, GABAARb3, GABRB3

Entrez Gene IDs

2562 (Human); 24922 (Rat)

Gene Symbol

GABRB3

UniProt

Additional GABA-A R beta 3 Products

Product Documents for GABA-A R beta 3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GABA-A R beta 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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