GABA-A R beta 3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-199
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Fusion protein from the cytoplasmic loop of the beta 3 subunit. Accession # P63079
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 21901840).
Specificity
Specific for the ~53 kDa beta 3-subunit of the GABAA receptor in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GABA-A R beta 3 Antibody
Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]
Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Immunostaining of mouse retina showing specific labeling of the GABAA beta3 subunit in green, calbindin in red and DNA in blue.Western Blot: GABA-A R beta 3 Antibody [NB300-199]
Western Blot: GABA-A R beta 3 Antibody [NB300-199] - Western blot of whole-olfactory bulb homogenates using the B3-subunit antibody in rAAV-Cre-injected versus non-injected GABRB3loxP/loxP mice (each six samples from three mice). The B3-subunit appears as a band of 55 kDa, consistent with its predicted molecular weight. Densitometric analysis (mean+/-s.e.m.) revealed a significant difference (t-test; *P=0.016). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms9950) licensed under a CC-BY license.Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]
Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Each channel was filtered frame by frame. The raw data was acquired at high confocal resolution (XY: 234.32 um x 234.32 um, pixel size = 0.114 um) using a 63x glycerol objective (NA=1.3). Z-steps were 0.15 um per frame. Acquired raw data (1) was filtered using an anisotropic diffusion 2D filter (2). (5). The final images (6) were obtained by thresholding the immunosignal. Scale bars: 10um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms9950) licensed under a CC-BY license.Applications for GABA-A R beta 3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:300
Simple Western
reported by internal validation
Western Blot
1:1000
Application Notes
Use in Immunohistochemistry free-floating reported in scientific literature (PMID: 26592770). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 17021187). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23430456). In Simple Western internal validation: Human Brain lysate at 0.5 mg/ml as sample; separated by size; antibody dilution of 1:100; observed molecular weight was 48 kDa;matrix was 12-230 kDa; detected by Chemiluminescence.
Reviewed Applications
Read 2 reviews rated 4.5 using NB300-199 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/ml BSA, 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GABA-A R beta 3
Long Name
gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide
Alternate Names
GABAAR beta 3, GABAARb3, GABRB3
Gene Symbol
GABRB3
UniProt
Additional GABA-A R beta 3 Products
Product Documents for GABA-A R beta 3 Antibody
Product Specific Notices for GABA-A R beta 3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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