GABAA R  gamma2 C-Terminus Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS029

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Primate

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all GABA-A R gamma 2 Antibodies »

Product Specifications

Immunogen

Fusion protein from the cytosolic loop of the rat GABAA R gamma2 subunit, C-Terminus

Specificity

Human, mouse, rat, and bovine ~44 - 47 kDa GABAA R gamma2 subunit, C-Terminus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GABAA R  gamma2 C-Terminus Antibody

Detection of GABAAR gamma2 C-Terminus Western Blot.

Detection of GABAAR gamma2 C-Terminus Western Blot.

Western blot of rat hippocampal lysate. The blot was incubated overnight at 2 - 8° C with anti-GABAAR gamma2 subunit, C-Terminus diluted 1:1000. The antibody labeled the ~44 - 47 kDa gamma2 subunit of the GABAAR.

Applications for GABAA R  gamma2 C-Terminus Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain (hippocampus) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R gamma 2

GABAA ( gamma-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, delta, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat gamma2 isoform is a 48 kDa, 436 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 30 - 233). The gamma2 subunit is part of the most common GABAA receptor combination in the mammalian brain ( alpha1 beta2 gamma2). GABA binds at alpha-beta interfaces, while benzodiazepine binds to alpha-gamma interfaces. There are two splice forms, the longest contains a consensus phosphorylation site in the second cytoplasmic domain, and a short form that shows an absence of this site through a deletion of aa 376 - 383. PKC phosphorylates the long form at S381, while both the short and long forms are phosphorylated at S365. Phosphorylation blocks receptor activity. The gamma2 subunits are also palmitoylated at multiple sites on cysteines that lay between aa 415 - 461, facilitating membrane trafficking.

References

  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119.
  4. Krishek, B.J. et al. (1994) Neuron 12:1081.
  5. Keller, C.A. et al. (2004) J. Neurosci. 24:5881.
  6. Moss, S.J. et al. (1992) J. Biol. Chem. 267:14470.

Long Name

gamma-Aminobutyric Acid Type A Receptor, gamma-2 Polypeptide

Alternate Names

GABAAR gamma 2, GABAARg2, GABRG2

Entrez Gene IDs

2566 (Human); 14406 (Mouse); 29709 (Rat); 282240 (Bovine)

Gene Symbol

GABRG2

Additional GABA-A R gamma 2 Products

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Product Datasheets

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Product Specific Notices for GABAA R  gamma2 C-Terminus Antibody

For research use only

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