Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S399-19

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GABA-A R alpha 2 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 350-385 (Cytoplasmic C-terminus) of rat GABA-A Receptor Alpha2

Localization

Cytoplasm

Specificity

Detects 55kDa. Does not cross-react with GABA-A Receptor Alpha1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GABA-AR alpha 2 Antibody (S399-19)

Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325]

Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325]

Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Western Blot analysis of Rat Brain showing detection of ~55 kDa GABA-AR alpha 2 Alpha 2 protein using Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 ug. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~55 kDa.
Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325]

Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325]

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 2 Antibody (D) Composite.
Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325]

Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325]

Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA-AR alpha 2 Alpha 2 protein using Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa. Other Band(s): ~ 37 kDa, ~50 kDa, ~70 kDa.
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Applications for GABA-AR alpha 2 Antibody (S399-19)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: GABA-A R alpha 2

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-2 Polypeptide

Alternate Names

GABAAR alpha 2, GABAARa2, GABRA2

Gene Symbol

GABRA2

Additional GABA-A R alpha 2 Products

Product Documents for GABA-AR alpha 2 Antibody (S399-19)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GABA-AR alpha 2 Antibody (S399-19)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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