GABARAPL3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18246
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide from the C-terminal of Human GABARAPL3
Localization
Cytoplasm|Cytoskeleton|Cytoplasmic Vesicle|Autophagosome Membrane|Lipid-Anchor
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GABARAPL3 Antibody
Western Blot: GABARAPL3 Antibody [NBP3-18246]
Western Blot: GABARAPL3 Antibody [NBP3-18246] - Western blot analysis of Rat Liver cell lysates showing detection of ~14 kDa GABARAPL3 protein using Rabbit Anti-GABARAPL3 Polyclonal Antibody (NBP3-18246). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL3 Polyclonal Antibody (NBP3-18246) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~14 kDa.Immunocytochemistry/ Immunofluorescence: GABARAPL3 Antibody [NBP3-18246]
Immunocytochemistry/Immunofluorescence: GABARAPL3 Antibody [NBP3-18246] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL3 Polyclonal Antibody (NBP3-18246). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL3 Polyclonal Antibody (NBP3-18246) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL3 Antibody (D) Composite.Applications for GABARAPL3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS, 50% glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GABARAPL3
Alternate Names
ATG8D, GABARAPL3, Gamma-aminobutyric acid receptor-associated protein-like 3
Gene Symbol
GABARAPL3
Additional GABARAPL3 Products
Product Documents for GABARAPL3 Antibody
Product Specific Notices for GABARAPL3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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