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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Summary for GABARAPL3 Antibody

Immunogen

Synthetic peptide from the C-terminal of Human GABARAPL3

Localization

Cytoplasm|Cytoskeleton|Cytoplasmic Vesicle|Autophagosome Membrane|Lipid-Anchor

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GABARAPL3 Antibody

Western Blot: GABARAPL3 Antibody [NBP3-18246]

Western Blot: GABARAPL3 Antibody [NBP3-18246]

Western Blot: GABARAPL3 Antibody [NBP3-18246] - Western blot analysis of Rat Liver cell lysates showing detection of ~14 kDa GABARAPL3 protein using Rabbit Anti-GABARAPL3 Polyclonal Antibody (NBP3-18246). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL3 Polyclonal Antibody (NBP3-18246) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~14 kDa.
Immunocytochemistry/ Immunofluorescence: GABARAPL3 Antibody [NBP3-18246]

Immunocytochemistry/ Immunofluorescence: GABARAPL3 Antibody [NBP3-18246]

Immunocytochemistry/Immunofluorescence: GABARAPL3 Antibody [NBP3-18246] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL3 Polyclonal Antibody (NBP3-18246). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL3 Polyclonal Antibody (NBP3-18246) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL3 Antibody (D) Composite.

Applications for GABARAPL3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS, 50% glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABARAPL3

Alternate Names

ATG8D, GABARAPL3, Gamma-aminobutyric acid receptor-associated protein-like 3

Gene Symbol

GABARAPL3

Additional GABARAPL3 Products

Product Documents for GABARAPL3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GABARAPL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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