GAD1/GAD67 Antibody (4I8S8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15480

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15480-100ul
NBP3-15480-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4I8S8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GAD1/GAD67 Antibodies »

Product Summary for GAD1/GAD67 Antibody (4I8S8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GAD1/GAD67 (Q99259). MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GAD1/GAD67 Antibody (4I8S8)

Western Blot: GAD1/GAD67 Antibody (4I8S8) [NBP3-15480]

Western Blot: GAD1/GAD67 Antibody (4I8S8) [NBP3-15480]

Western Blot: GAD1/GAD67 Antibody (4I8S8) [NBP3-15480] - Western blot analysis of extracts of various cell lines, using GAD1/GAD67 Rabbit mAb (NBP3-15480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: GAD1/GAD67 Antibody (4I8S8) [NBP3-15480]

Western Blot: GAD1/GAD67 Antibody (4I8S8) [NBP3-15480]

Western Blot: GAD1/GAD67 Antibody (4I8S8) [NBP3-15480] - Western blot analysis of extracts of various cell lines, using GAD1/GAD67 Rabbit mAb (NBP3-15480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for GAD1/GAD67 Antibody (4I8S8)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Gene Symbol

GAD1

Additional GAD1/GAD67 Products

Product Documents for GAD1/GAD67 Antibody (4I8S8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAD1/GAD67 Antibody (4I8S8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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