GAD1/GAD67 Antibody (HL1096)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13716

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-13716
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Feline, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1096

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GAD1/GAD67 Antibodies »

Product Summary for GAD1/GAD67 Antibody (HL1096)

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAD1/GAD67. The exact sequence is proprietary.

Reactivity Notes

Cat (100%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for GAD1/GAD67 Antibody (HL1096)

Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]

Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]

Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - GAD1/GAD67 antibody [HL1096] detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAD1/GAD67 stained by GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - GAD1/GAD67 antibody [HL1096] detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: GAD1/GAD67 stained by GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a nerual marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for GAD1/GAD67 Antibody (HL1096)

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Gene Symbol

GAD1

Additional GAD1/GAD67 Products

Product Documents for GAD1/GAD67 Antibody (HL1096)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAD1/GAD67 Antibody (HL1096)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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