Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Zebrafish

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3G9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all GAD1/GAD67 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GAD1 (NP_000808) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

66.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free

Western Blot: GAD1/GAD67 Antibody (OTI3G9)Azide and BSA Free [NBP2-70791]

Western Blot: GAD1/GAD67 Antibody (OTI3G9)Azide and BSA Free [NBP2-70791]

Western Blot: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1/GAD67 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1/GAD67.
Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]

Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]

Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of A549 cells using anti-GAD1/GAD67 mouse monoclonal antibody.
Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]

Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791]

Immunohistochemistry: GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free [NBP2-70791] - Staining of paraffin-embedded Human pancreas tissue using anti-GAD1/GAD67 mouse monoclonal antibody.
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Applications for GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Immunoprecipitation

4ug/ml

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Gene Symbol

GAD1

Additional GAD1/GAD67 Products

Product Documents for GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GAD1/GAD67 Antibody (OTI3G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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