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GAD1/GAD67 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-02161

Novus Biologicals, part of Bio-Techne
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NBP1-02161

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

0.1 mg/ml

Product Specifications

Immunogen

Chickens were immunized with a synthetic peptide/keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a region near the C-terminus of GAD1/GAD67, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products.

Marker

Neuron Cell Marker

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAD1/GAD67 Antibody

Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161]

Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161]

Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161] - Immunocytochemical staining of GAD1/GAD67 (RED/YELLOW) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD1/GAD67 staining was detected using Texas Red goat anti-chicken IgY. MAP2 staining (GREEN) used a rabbit antibody.
Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161]

Immunocytochemistry/ Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161]

Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161] - Immunohistochemical staining of inhibitory neurons (RED) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The brains were fixed with 4.0% paraformaldehyde. The green staining is low level GFP fluorescence expressed off the actin promoter in this mouse transgenic strain.
GAD1/GAD67 Antibody Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161]

Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161]

Immunocytochemical staining of GAD1/GAD67 (red/yellow) in cultured cortical neurons from a neonatal mouse (C57/Black6) using Texas Red goat anti-chicken IgY as a secondary (1:500).

Applications for GAD1/GAD67 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry

1:500-1:1000

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10mM PBS (0.9% isotonic, w/v, pH 7.2)

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GAD1/GAD67

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Entrez Gene IDs

2571 (Human); 14415 (Mouse); 24379 (Rat)

Gene Symbol

GAD1

UniProt

Additional GAD1/GAD67 Products

Product Documents for GAD1/GAD67 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAD1/GAD67 Antibody

Chicken products cannot be exported to Canada.

Purification Notes
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation was filter-sterilized through a 0.45 um filter.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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