GAD2/GAD65 Antibody (5D9G12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52471

Novus Biologicals, part of Bio-Techne
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NBP2-52471
NBP2-52471-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5D9G12

Format

BSA Free

Concentration

1 mg/ml

View all GAD2/GAD65 Antibodies »

Product Summary for GAD2/GAD65 Antibody (5D9G12) - BSA Free

Immunogen

Purified recombinant fragment of human GAD2/GAD65 (AA: 1-100) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

65.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAD2/GAD65 Antibody (5D9G12) - BSA Free

Western Blot: GAD2/GAD65 Antibody (5D9G12)BSA Free [NBP2-52471]

Western Blot: GAD2/GAD65 Antibody (5D9G12)BSA Free [NBP2-52471]

Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: GAD2/GAD65 Antibody (5D9G12) - BSA Free [NBP2-52471]

Flow Cytometry: GAD2/GAD65 Antibody (5D9G12) - BSA Free [NBP2-52471]

Flow Cytometry: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis of Hela cells using GAD2 mouse mAb (green) and negative control (red).
Western Blot: GAD2/GAD65 Antibody (5D9G12)BSA Free [NBP2-52471]

Western Blot: GAD2/GAD65 Antibody (5D9G12)BSA Free [NBP2-52471]

Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against human GAD2 (AA: 1-100) recombinant protein. (Expected MW is 36.3 kDa)
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Applications for GAD2/GAD65 Antibody (5D9G12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD2/GAD65

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Gene Symbol

GAD2

Additional GAD2/GAD65 Products

Product Documents for GAD2/GAD65 Antibody (5D9G12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAD2/GAD65 Antibody (5D9G12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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