GAD2/GAD65 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1506

Novus Biologicals, part of Bio-Techne
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NB100-1506
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all GAD2/GAD65 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TLEDNEERMSRLSK corresponding to internal region according to NP_000809.1, NP_001127838.1.

Localization

Cytoplasmic

Specificity

This antibody is expected to recognise both reported variants.

Marker

GABAergic Neuronal Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GAD2/GAD65 Antibody

Western Blot: GAD2/GAD65 Antibody [NB100-1506]

Western Blot: GAD2/GAD65 Antibody [NB100-1506]

Western Blot: GAD2/GAD65 Antibody [NB100-1506] - (0.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
GAD2/GAD65 Antibody

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NB100-1506] -

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NB100-1506] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
GAD2/GAD65 Antibody

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NB100-1506] -

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NB100-1506] - (6ug/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for GAD2/GAD65 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

6-8 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5-1.5 ug/ml
Application Notes
WB: Approx 65kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Primary incubation was 1 hour.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GAD2/GAD65

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Entrez Gene IDs

2572 (Human)

Gene Symbol

GAD2

UniProt

NP_000809.1. (Human)

Additional GAD2/GAD65 Products

Product Documents for GAD2/GAD65 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GAD2/GAD65 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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