GADD153/CHOP Antibody (S02-6A3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14934

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14934-25ul
NBP3-14934-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-6A3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GADD153 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human GADD153/CHOP (Uniprot#: P35638)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GADD153/CHOP Antibody (S02-6A3)

Western Blot: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934]

Western Blot: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934]

Western Blot: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934] - Western blot detection of GADD153/CHOP in K562, CHO-K1, Hela cell lysates using GADD153/CHOP Rabbit mAb (1:1000 diluted). Predicted band size: 19KDa. Observed band size: 27KDa.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934]

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934] - Immunohistochemistry of GADD153/CHOP in paraffin-embedded Human breast cancer tissue using GADD153/CHOP Rabbit mAb at dilution 1/1
Western Blot: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934]

Western Blot: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934]

Western Blot: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934] - Western blot detection of GADD153/CHOP in 3T3 cell lysates using GADD153/CHOP Rabbit mAb (1:1000 diluted). Predicted band size: 19KDa. Observed band size: 27KDa.

Applications for GADD153/CHOP Antibody (S02-6A3)

Application
Recommended Usage

Immunohistochemistry

1/20-1/50

Immunohistochemistry-Paraffin

1/20-1/50

Western Blot

1/1000-1/2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GADD153

GADD153 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Long Name

Growth Arrest and DNA Damage-inducible Protein GADD153

Alternate Names

CEBP zeta, CHOP, CHOP10, DDIT3

Gene Symbol

DDIT3

Additional GADD153 Products

Product Documents for GADD153/CHOP Antibody (S02-6A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GADD153/CHOP Antibody (S02-6A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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