GADD153/CHOP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98741

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GADD153 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human GADD153/CHOP.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for GADD153/CHOP Antibody

Immunocytochemistry/ Immunofluorescence: GADD153/CHOP Antibody [NBP2-98741]

Immunocytochemistry/ Immunofluorescence: GADD153/CHOP Antibody [NBP2-98741]

Immunocytochemistry/Immunofluorescence: GADD153/CHOP Antibody [NBP2-98741] - Immunofluorescence staining of GADD153/CHOP in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum. Then incubated with rabbit anti-human GADD153/CHOP polyclonal antibody (1:1000)(Figure A), incubated with rabbit anti-human GADD153/CHOP polyclonal antibody and antigen (Figure B) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.

Applications for GADD153/CHOP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GADD153

GADD153 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Long Name

Growth Arrest and DNA Damage-inducible Protein GADD153

Alternate Names

CEBP zeta, CHOP, CHOP10, DDIT3

Gene Symbol

DDIT3

Additional GADD153 Products

Product Documents for GADD153/CHOP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GADD153/CHOP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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