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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1C9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Summary for GADD45 alpha Antibody (OTI1C9) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human GADD45A(NP_001915) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

18.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GADD45 alpha Antibody (OTI1C9) - Azide and BSA Free

Western Blot: GADD45 alpha Antibody (OTI1C9)Azide and BSA Free [NBP2-70559]

Western Blot: GADD45 alpha Antibody (OTI1C9)Azide and BSA Free [NBP2-70559]

Western Blot: GADD45 alpha Antibody (OTI1C9) - Azide and BSA Free [NBP2-70559] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A.

Applications for GADD45 alpha Antibody (OTI1C9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GADD45 alpha

GADD45A is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. [provided by RefSeq]

Long Name

Growth Arrest and DNA Damage Gene alpha

Alternate Names

GADD45a

Gene Symbol

GADD45A

Additional GADD45 alpha Products

Product Documents for GADD45 alpha Antibody (OTI1C9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GADD45 alpha Antibody (OTI1C9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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