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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 675 to 725 of human Cyclin G Associated Kinase using the numbering given in entry NP_005246.2 (GeneID 2580).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GAK Antibody

Western Blot: GAK Antibody [NBP2-32181]

Western Blot: GAK Antibody [NBP2-32181]

Western Blot: GAK Antibody [NBP2-32181] - Whole cell lysate (10 ug) from CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAK antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: GAK Antibody [NBP2-32181]

Western Blot: GAK Antibody [NBP2-32181]

Western Blot: GAK Antibody [NBP2-32181] - Whole cell lysate (10 ug) from GaMG, HeLa, HEK293T, Jurkat, andRKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAK antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for GAK Antibody

Application
Recommended Usage

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GAK

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5.

Long Name

Cyclin G-associated Kinase

Alternate Names

Auxillin 2

Gene Symbol

GAK

Additional GAK Products

Product Documents for GAK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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