Galactosylceramidase/GALC Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16578
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rhesus Macaque (95%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Galactosylceramidase/GALC. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Canine (87%), Bovine (84%), Pig (87%).
Localization
Lysosome
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Galactosylceramidase/GALC Antibody
![Immunohistochemistry-Paraffin: Galactosylceramidase/GALC Antibody [NBP2-16578]](https://resources.bio-techne.com/images/products/Galactosylceramidase-GALC-Antibody-Immunohistochemistry-Paraffin-NBP2-16578-img0003.jpg "Immunohistochemistry-Paraffin: Galactosylceramidase/GALC Antibody [NBP2-16578]")
Immunohistochemistry-Paraffin: Galactosylceramidase/GALC Antibody [NBP2-16578]
Immunohistochemistry-Paraffin: Galactosylceramidase/GALC Antibody [NBP2-16578] - Human ovarian cancer diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.![Western Blot: Galactosylceramidase/GALC Antibody [NBP2-16578]](https://resources.bio-techne.com/images/products/Galactosylceramidase-GALC-Antibody-Western-Blot-NBP2-16578-img0002.jpg "Western Blot: Galactosylceramidase/GALC Antibody [NBP2-16578]")
Western Blot: Galactosylceramidase/GALC Antibody [NBP2-16578]
Western Blot: Galactosylceramidase/GALC Antibody [NBP2-16578] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Galactosylceramidase antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Galactosylceramidase/GALC Antibody [NBP2-16578] -
Galactosylceramidase/GALC antibody detects Galactosylceramidase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.Galactosylceramidase stained by Galactosylceramidase antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Applications for Galactosylceramidase/GALC Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500 - 1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Galactosylceramidase/GALC
Alternate Names
Galactocerebrosidase, Galactocerebroside beta-galactosidase, Galactosylceraminidase, GALC
Gene Symbol
GALC
UniProt
Additional Galactosylceramidase/GALC Products
Product Documents for Galactosylceramidase/GALC Antibody
Product Specific Notices for Galactosylceramidase/GALC Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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