Galactosylceramidase/GALC Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03718

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Galactosylceramidase/GALC Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human Galactosylceramidase/GALC (NP_000144.2). GGAYVLDDSDGLGREFDGIGAVSGGGATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Galactosylceramidase/GALC Antibody - BSA Free

Western Blot: Galactosylceramidase/GALC AntibodyBSA Free [NBP3-03718]

Western Blot: Galactosylceramidase/GALC AntibodyBSA Free [NBP3-03718]

Western Blot: Galactosylceramidase/GALC Antibody [NBP3-03718] - Analysis of extracts of various cell lines, using Galactosylceramidase/GALC antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/ Immunofluorescence: Galactosylceramidase/GALC Antibody - BSA Free [NBP3-03718]

Immunocytochemistry/ Immunofluorescence: Galactosylceramidase/GALC Antibody - BSA Free [NBP3-03718]

Immunocytochemistry/Immunofluorescence: Galactosylceramidase/GALC Antibody [NBP3-03718] - Analysis of HeLa cells using GALC Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Galactosylceramidase/GALC Antibody - BSA Free [NBP3-03718]

Immunocytochemistry/ Immunofluorescence: Galactosylceramidase/GALC Antibody - BSA Free [NBP3-03718]

Immunocytochemistry/Immunofluorescence: Galactosylceramidase/GALC Antibody [NBP3-03718] - Analysis of A-549 cells using GALC Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
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Applications for Galactosylceramidase/GALC Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Galactosylceramidase/GALC

The GALC gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as

Alternate Names

Galactocerebrosidase, Galactocerebroside beta-galactosidase, Galactosylceraminidase, GALC

Gene Symbol

GALC

Additional Galactosylceramidase/GALC Products

Product Documents for Galactosylceramidase/GALC Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Galactosylceramidase/GALC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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