Galanin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45217

Novus Biologicals, part of Bio-Techne
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NBP1-45217
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Frog - Xenopus (African Clawed Frog)

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Galanin Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HRSFSDKNGLTSK corresponding to internal region according to NP_057057.2.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Galanin Antibody

Western Blot: Galanin Antibody [NBP1-45217]

Western Blot: Galanin Antibody [NBP1-45217]

Western Blot: Galanin Antibody [NBP1-45217] - Staining of Mouse (A) and Rat (B) Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: Galanin Antibody [NBP1-45217]

Western Blot: Galanin Antibody [NBP1-45217]

Western Blot: Galanin Antibody [NBP1-45217] - Analysis of Galanin in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-45217 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Galanin Antibody

Application
Recommended Usage

Immunohistochemistry-Frozen

0.2 - 0.5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 17 kDa band observed in human brain (cerebellum) and in mouse and rat brain lysates (calculated MW of 13.3 kDa band according to human NP_057057.2 and of 13.5 kDa band according to mouse NP_034383.1 and rat NP_150240.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Galanin

Galanin is a 29/30 amino acid neuropeptide widely distributed in the peripheral and central nervous systems and endocrine system. The amino acid sequence of galanin is very conserved (almost 90% among species), indicating the importance of the molecule. In the brain, the highest concentrations are observed in the hypothalamus and particularly in the nerve terminals of the median eminence. There are three well-characterized G-protein coupled galanin receptors subtypes (GalR1-3). A more recently discovered 60 amino acid galanin-like peptide (GALP) shares amino acid sequence homology with galanin (1-13) in position 9-21 and has high binding affinity for GalR1-3, with highest affinity for GalR3. The fact that Galanin has a wide spectrum of activities indicates that it is an important messenger for intercellular communication within the nervous system and the neuroendocrine axis. Both galanin and GALP are implicated in the regulation of food and water intake behavior, and many related neuroendocrine functions.

Alternate Names

GAL, GALN

Entrez Gene IDs

51083 (Human); 14419 (Mouse); 29141 (Rat)

Gene Symbol

GAL

UniProt

P22466 (Human)

Additional Galanin Products

Product Documents for Galanin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Galanin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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