Galectin-3 Antibody (024)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89383

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-89383-100ul
NBP2-89383-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 024

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Galectin-3 Antibodies »

Product Summary for Galectin-3 Antibody (024)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Galectin-3 (Accession#: P17931; Met1-Ile250).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Galectin-3 Antibody (024)

Western Blot: Galectin-3 Antibody (024) [NBP2-89383]

Western Blot: Galectin-3 Antibody (024) [NBP2-89383]

Western Blot: Galectin-3 Antibody (024) [NBP2-89383] - Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa
Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (024) [NBP2-89383]

Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (024) [NBP2-89383]

Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (024) [NBP2-89383] - Staining of Human Galectin3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human Galectin3 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nuclear and cytoplasm.
Flow Cytometry: Galectin-3 Antibody (024) [NBP2-89383]

Flow Cytometry: Galectin-3 Antibody (024) [NBP2-89383]

Flow Cytometry: Galectin-3 Antibody (024) [NBP2-89383] - Analysis of Human Galectin3 expression on HeLa cells. The cells were treated stained with purified anti-Human Galectin3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Applications for Galectin-3 Antibody (024)

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

0.5-2uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Galectin-3

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Gene Symbol

LGALS3

Additional Galectin-3 Products

Product Documents for Galectin-3 Antibody (024)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Galectin-3 Antibody (024)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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