Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 5C21

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Galectin-3 Antibodies »

Product Specifications

Immunogen

Full length recombinant human Galectin-3 expressed in and purified from E. coli. [UniProt# P17931]

Localization

Cytoplasm. Nucleus. Secreted. Note: Secreted by a non-classical secretory pathway and associates with the cell surface.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for Galectin-3 Antibody (5C21) [DyLight 405]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Galectin-3 (5C21) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 30 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Galectin-3

Galectin-3 (LGALS3 or Mac-2 antigen), is a 26 kD beta-galactoside-binding protein from galectin family and it is ubiquitously expressed with localization dependent on cell type as well as proliferative status. Galectin-3 is expressed in many immunocompetent and inflammatory cells, including macrophages, dendritic cells, eosinophils, mast cells, uterine NK cells and activated T and B cells, and it is localized in nucleus, cytoplasm or on cell surface as well as in extracellular compartments. Extracellularly, galectin-3 acts as an adhesion molecule whereas intracellular galectin-3 implicates in processes that are pivotal for basic cellular functions, such as pre-mRNA splicing regulation of cell growth, cell cycle progression and apoptosis. Galectin-3 binds and interacts with numerous ligands in intra-/extracellular environment such as DMBT1, ITGA3, ITGB1, CSPG4, LGALS3BP, LYPD3, CYHR1, UACA etc. for modulating cellular signals responsible fpr its biological functions such as phagocytosis, cell growth/proliferation, adhesion and apoptosis in normal as well as cancer cells. Circulating galectin-3 concentrations increases more than 30-fold in bloodstream of patients with various cancers and patients with metastatic disease have higher concentrations of circulating galectin-3 than those with only localized tumors.

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Gene Symbol

LGALS3

Additional Galectin-3 Products

Product Documents for Galectin-3 Antibody (5C21) [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Galectin-3 Antibody (5C21) [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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