Galectin-3 Antibody (GT12712)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13641
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT12712
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Galectin-3. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Galectin-3 Antibody (GT12712)
![Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641]](https://resources.bio-techne.com/images/products/Galectin-3-Antibody-GT12712-Western-Blot-NBP3-13641-img0004.jpg "Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641]")
Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641]
Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641] - Wild-type (WT) and Galectin-3 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (GT12712) [NBP3-13641]](https://resources.bio-techne.com/images/products/Galectin-3-Antibody-GT12712-Immunocytochemistry-Immunofluorescence-NBP3-13641-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (GT12712) [NBP3-13641]")
Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (GT12712) [NBP3-13641]
Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (GT12712) [NBP3-13641] - Galectin-3 antibody [GT12712] detects Galectin-3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Galectin-3 stained by Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar= 10 um.![Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641]](https://resources.bio-techne.com/images/products/Galectin-3-Antibody-GT12712-Western-Blot-NBP3-13641-img0001.jpg "Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641]")
Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641]
Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Applications for Galectin-3 Antibody (GT12712)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Galectin-3
Alternate Names
AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2
Gene Symbol
LGALS3
Additional Galectin-3 Products
Product Documents for Galectin-3 Antibody (GT12712)
Product Specific Notices for Galectin-3 Antibody (GT12712)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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