Galectin-4 Antibody (MM0300-1D12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12323

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # MM0300-1D12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Galectin-4 Antibodies »

Product Summary for Galectin-4 Antibody (MM0300-1D12) - Azide and BSA Free

Immunogen

Human recombinant Galectin-4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Scientific Data Images for Galectin-4 Antibody (MM0300-1D12) - Azide and BSA Free

Immunohistochemistry-Paraffin: Galectin-4 Antibody (MM0300-1D12) - Azide and BSA Free [NBP2-12323]

Immunohistochemistry-Paraffin: Galectin-4 Antibody (MM0300-1D12) - Azide and BSA Free [NBP2-12323]

Immunohistochemistry-Paraffin: Galectin-4 Antibody (MM0300-1D12) [NBP2-12323] - 4% PFA fixed and parffine embedded human placental tissue section (4um) is subjected to IHC staining. Tissue was treated with 15ug/ml Protenase K for antige retrieval before IHC is applied.

Applications for Galectin-4 Antibody (MM0300-1D12) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

15 ug/ml

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Galectin-4

Galectins are a family of soluble beta-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One member of this family, Galectin-4, also known as Gal-4, L36 or LGALS4 maps to human chromosome 19q13.13 and encodes a 36-37 kDa protein. The Galectin-4 protein is composed of 323 amino acids and contains two homologous carbohydrate recognition domains (CRD) and all amino acids typically conserved in the galectin family. Expression of Galectin-4 correlates with the malignant potential of human hepatocellular carcinoma (HCC) and is differentially regulated depending on cell-cell contact, serum growth factors, cell growth and cell differentiation status. Galectin-4 expression is detected in epithelial cells of the colon, rectum, intestine, and in HT29 and LS174T cell lines. Galectin-4 is underexpressed in colorectal cancer and is preferentially upregulated in cells prone to peritoneal dissemination.

Alternate Names

GAL4, Galectin4, LGALS4

Gene Symbol

LGALS4

UniProt

P56470

Additional Galectin-4 Products

Product Documents for Galectin-4 Antibody (MM0300-1D12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Galectin-4 Antibody (MM0300-1D12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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