Galectin-8 Antibody (106.1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11211

Novus Biologicals, part of Bio-Techne
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NBP3-11211
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Key Product Details

Species Reactivity

Human, Rat

Applications

Flow Cytometry, Functional, Immunoassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 106.1

Concentration

0.1 mg/ml

View all Galectin-8 Antibodies »

Product Specifications

Immunogen

Recombinant rat Galectin-8

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<24 EU/mg

Applications for Galectin-8 Antibody (106.1)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Flow Cytometry and Functional Studies dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.-

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Galectin-8

Galectins are animal lectins that can specifically bind beta-galactosides. Thirteen galectins have already been described, belonging to three different groups: prototype, tandem-repeat and chimeric. Galectins are involved in cellular interactions and neoplastic transformations. Galectin-8, is a 34 kDa protein made of tandem-repeat carbohydrate recognition domains (CRDs), joined by a "link" peptide. Structurally, galectin-8 belongs to the tandem-repeat type galectins. The galectin-8 gene ( LGALS8) encodes numerous mRNAs by alternate splicing and the presence of three unusual polyadenylation signals. These mRNAs could encode for six isoforms of galectin-8, of which three belong to the tandem-repeat galectin group (with two carbohydrate binding domains) and the three others to the prototype group (one carbohydrate binding domain). Galectin-8, like other galectins, is a secreted protein. Upon secretion galectin-8 acts as a physiological modulator of cell adhesion. When immobilized, it functions as a matrix protein equipotent to fibronectin in promoting cell adhesion by ligation and clustering of a selective subset of cell surface integrin receptors. Although galectin-8 is a fairly abundant protein, it is not ubiquitously expressed. It shows a micro-clustering pattern reminiscent of that seen with proteins associated with mitochondria, the Golgi or trans-Golgi membranes. Studies of galectin-8 revealed that galectin-8 positively or negatively regulates cell adhesion, depending on the extracellular context. Cell adhesion to galectin-8 triggers integrin-mediated signalling cascades such as Tyr phosphorylation of FAK and paxillin. Galectin-8 is widely expressed in normal tissues (brain, breast, colon, retina, kidney, pancreas, placenta, spleen, testis, uterus, vascular, esophagus and heart) as well as in tumor tissues (brain, breast, colon, germ cells, head and neck, kidney, muscles, ovary, pancreas, thyroid, placenta, prostate, uterus, lung, stomach and esophagus)."

Alternate Names

GAL8, Galectin8, LGALS8, PCTA1

Gene Symbol

LGALS8

Additional Galectin-8 Products

Product Documents for Galectin-8 Antibody (106.1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Galectin-8 Antibody (106.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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