Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1D12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Galectin-9 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LGALS9 (NP_002299) produced in HEK293T cell.

Reactivity Notes

Mouse reactivity reported in (PMID: 29316433). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

35.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Galectin-9 Antibody (OTI1D12) - Azide and BSA Free

Western Blot: Galectin-9 Antibody (OTI1D12)Azide and BSA Free [NBP2-71132]

Western Blot: Galectin-9 Antibody (OTI1D12)Azide and BSA Free [NBP2-71132]

Western Blot: Galectin-9 Antibody (OTI1D12) - Azide and BSA Free [NBP2-71132] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Galectin-9.
Immunohistochemistry: Galectin-9 Antibody (OTI1D12) - Azide and BSA Free [NBP2-71132]

Immunohistochemistry: Galectin-9 Antibody (OTI1D12) - Azide and BSA Free [NBP2-71132]

Immunohistochemistry: Galectin-9 Antibody (OTI1D12) - Azide and BSA Free [NBP2-71132] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Galectin-9 Antibody (OTI1D12) - Azide and BSA Free [NBP2-71132]

Immunohistochemistry: Galectin-9 Antibody (OTI1D12) - Azide and BSA Free [NBP2-71132]

Immunohistochemistry: Galectin-9 Antibody (OTI1D12) - Azide and BSA Free [NBP2-71132] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for Galectin-9 Antibody (OTI1D12) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Galectin-9

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 9 is an S-type lectin and an eosinophil chemoattractant (ECA) produced by antigen-activated T cells. Galectin 9 is involved in apoptosis, is thought to be a prognostic factor for breast cancer and is also overexpressed in Hodgkin's disease tissue. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide. Multiple alternatively spliced transcript variants have been found for this gene.

Alternate Names

Ecalectin, GAL9, Galectin9, LGALS9

Gene Symbol

LGALS9

Additional Galectin-9 Products

Product Documents for Galectin-9 Antibody (OTI1D12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Galectin-9 Antibody (OTI1D12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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