GALT Antibody (PSH02-87)

Western Blot: GALT Antibody (PSH02-87) [NBP3-32363]

Western Blot: GALT Antibody (PSH02-87) [NBP3-32363] - Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (NBP3-32363) at 1/2,000 dilution.

Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: A549 cell lysate (20 ug/Lane)
Lane 4: K-562 cell lysate (20 ug/Lane)
Lane 5: SH-SY5Y cell lysate (20 ug/Lane)
Lane 6: PC-12 cell lysate (20 ug/Lane)
Lane 7: Rat liver tissue lysate (40 ug/Lane)

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32363) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: GALT Antibody (PSH02-87) [NBP3-32363]

Immunocytochemistry/ Immunofluorescence: GALT Antibody (PSH02-87) [NBP3-32363] - Immunocytochemistry analysis of HeLa cells labeling GALT with Rabbit anti-GALT antibody (NBP3-32363) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GALT antibody (NBP3-32363) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: GALT Antibody (PSH02-87) [NBP3-32363]

Flow Cytometry: GALT Antibody (PSH02-87) [NBP3-32363] - Flow cytometric analysis of HeLa cells labeling GALT.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32363, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).