gamma Catenin Antibody (15F11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53325
![Flow Cytometry: gamma Catenin Antibody (15F11) [NBP2-53325]](https://resources.bio-techne.com/images/products/gamma-Catenin-Antibody-15F11-Flow-Cytometry-NBP2-53325-img0002.jpg "Flow Cytometry: gamma Catenin Antibody (15F11) [NBP2-53325]")
![Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325]](https://resources.bio-techne.com/images/products/gamma-Catenin-Antibody-15F11-Western-Blot-NBP2-53325-img0001.jpg "Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325]")
![Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325]](https://resources.bio-techne.com/images/products/gamma-Catenin-Antibody-15F11-Western-Blot-NBP2-53325-img0004.jpg "Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Canine, Chicken, Feline
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 15F11
Concentration
0.2 mg/ml
Product Summary for gamma Catenin Antibody (15F11)
Immunogen
Recombinant, full length chicken gamma Catenin protein (Uniprot: P14923)
Localization
Cell Surface and Cytoplasmic
Marker
Cardiomyocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54527)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for gamma Catenin Antibody (15F11)
Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325]
Western Blot: gamma Catenin Antibody (15F11) [NBP2-53325] - Western Blot Analysis of human HeLa and SK-BR3 cell lysate using gamma Catenin Antibody (15F11).![Immunocytochemistry/ Immunofluorescence: gamma Catenin Antibody (15F11) [NBP2-53325]](https://resources.bio-techne.com/images/products/gamma-Catenin-Antibody-15F11-Immunocytochemistry-Immunofluorescence-NBP2-53325-img0003.jpg "Immunocytochemistry/ Immunofluorescence: gamma Catenin Antibody (15F11) [NBP2-53325]")
Immunocytochemistry/ Immunofluorescence: gamma Catenin Antibody (15F11) [NBP2-53325]
Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (15F11) [NBP2-53325] - Confocal immunofluorescence image of HeLa cells using gamma Catenin Antibody (15F11) Green (CF488) and Red Dot is used to label the nuclei Red.![Immunohistochemistry-Paraffin: gamma Catenin Antibody (15F11) [NBP2-53325]](https://resources.bio-techne.com/images/products/gamma-Catenin-Antibody-15F11-Immunohistochemistry-Paraffin-NBP2-53325-img0005.jpg "Immunohistochemistry-Paraffin: gamma Catenin Antibody (15F11) [NBP2-53325]")
Immunohistochemistry-Paraffin: gamma Catenin Antibody (15F11) [NBP2-53325]
Immunohistochemistry-Paraffin: gamma Catenin Antibody (15F11) [NBP2-53325] - Formalin-fixed, paraffin-embedded human cervix stained with gamma Catenin Antibody (15F11). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.Applications for gamma Catenin Antibody (15F11)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: gamma Catenin
Alternate Names
catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa, Catenin gamma, Desmoplakin III, desmoplakin-3, DP3CTNNG, DPIII, gamma-catenin, junction plakoglobin, PDGBARVD12, PKGB
Gene Symbol
JUP
UniProt
Additional gamma Catenin Products
Product Documents for gamma Catenin Antibody (15F11)
Product Specific Notices for gamma Catenin Antibody (15F11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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