gamma Catenin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1060

Novus Biologicals, part of Bio-Techne
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NB100-1060
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all gamma Catenin Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GLRPPYPTADHMLA corresponding to C-Terminus according to NP_002221.1, NP_068831.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine.

Localization

Cytoplasmic in a soluble and membrane-associated form.

Specificity

This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for gamma Catenin Antibody

Western Blot: gamma Catenin Antibody [NB100-1060]

Western Blot: gamma Catenin Antibody [NB100-1060]

Western Blot: gamma Catenin Antibody [NB100-1060] - Staining (1ug/ml) of Rat Eye lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: gamma Catenin Antibody [NB100-1060]

Immunohistochemistry-Paraffin: gamma Catenin Antibody [NB100-1060]

Immunohistochemistry-Paraffin: gamma Catenin Antibody [NB100-1060] - Staining of Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: gamma Catenin Antibody [NB100-1060]

Western Blot: gamma Catenin Antibody [NB100-1060]

Western Blot: gamma Catenin Antibody [NB100-1060] - Staining of A431 (A) and NIH3T3 (B) cell lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for gamma Catenin Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
EIA: An anonymous customer has reported successful usage quantitative ELISA assays (EIA), very sensitive to freeze/thaw cycles. WB: Approx. 85 kDa band observed in lysates of A431A 549 and NIH3T3 and in rat eye lysates (calculated MW of 81.7 kDa band according to human NP_002221.1 and mouse NP_034723.1).
IHC-Paraffin embedded Human Heart.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: gamma Catenin

Plakoglobin is a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described.

Alternate Names

catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa, Catenin gamma, Desmoplakin III, desmoplakin-3, DP3CTNNG, DPIII, gamma-catenin, junction plakoglobin, PDGBARVD12, PKGB

Entrez Gene IDs

3728 (Human); 16480 (Mouse)

Gene Symbol

JUP

UniProt

NP_002221 (Human)

Additional gamma Catenin Products

Product Documents for gamma Catenin Antibody

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Certificate of Analysis

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Product Specific Notices for gamma Catenin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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