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gamma Sarcoglycan Antibody (3C5)

Novus Biologicals, part of Bio-Techne | Catalog # H00006445-M02

Novus Biologicals, part of Bio-Techne
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H00006445-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3C5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SGCG (NP_000222.1, 191 a.a. ~ 290 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DLRLESPTRSLSMDAPRGVHIQAHAGKIEALSQMDILFHSSDGMLVLDAETVCLPKLVQGTWGPSGSSQSLYEICVCPDGKLYLSVAGVSTTCQEHSHIC

Specificity

SGCG - sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein) (3C5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for gamma Sarcoglycan Antibody (3C5)

Sandwich ELISA: gamma Sarcoglycan Antibody (3C5) [H00006445-M02] - Detection limit for recombinant GST tagged SGCG is 0.1 ng/ml as a capture antibody.
Immunoprecipitation: gamma Sarcoglycan Antibody (3C5) [H00006445-M02]

Immunoprecipitation: gamma Sarcoglycan Antibody (3C5) [H00006445-M02]

Immunoprecipitation: gamma Sarcoglycan Antibody (3C5) [H00006445-M02] - Analysis of SGCG transfected lysate using anti-SGCG monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SGCG MaxPab rabbit polyclonal antibody.

Applications for gamma Sarcoglycan Antibody (3C5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: gamma-Sarcoglycan/SGCG

Gamma-sarcoglycan is one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin, probably to provide a link between the membrane associated cytoskeleton and the extracellular matrix. Defects in the protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). [provided by RefSeq]

Long Name

Sarcoglycan, Gamma (35kDa Dystrophin-Associated Glycoprotein)

Alternate Names

DAGA4, DMDA, DMDA1, LGMD2C, LGMDR5, MGC130048, SCARMD2, SCG3

Entrez Gene IDs

6445 (Human)

Gene Symbol

SGCG

Additional gamma-Sarcoglycan/SGCG Products

Product Documents for gamma Sarcoglycan Antibody (3C5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for gamma Sarcoglycan Antibody (3C5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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