gamma Tubulin Antibody (9D6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60245

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 9D6

Format

BSA Free

Concentration

1 mg/ml

View all gamma Tubulin Antibodies »

Product Summary for gamma Tubulin Antibody (9D6) - BSA Free

Immunogen

Recombinant human gamma Tubulin (1-451aa) purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for gamma Tubulin Antibody (9D6) - BSA Free

Western Blot: gamma Tubulin Antibody (9D6)BSA Free [NBP2-60245]

Western Blot: gamma Tubulin Antibody (9D6)BSA Free [NBP2-60245]

Western Blot: gamma Tubulin Antibody (9D6) [NBP2-60245] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human gamma Tubulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysate Lane 3.: A549 cell lysate Lane 4.: K562 cell lysate
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (9D6) - BSA Free [NBP2-60245]

Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (9D6) - BSA Free [NBP2-60245]

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (9D6) [NBP2-60245] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Applications for gamma Tubulin Antibody (9D6) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gamma Tubulin

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The g-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing g-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Long Name

Tubulin gamma-1 chain

Alternate Names

Gamma-1-tubulin, GCP-1, TUBG, TUBG1

Gene Symbol

TUBG1

Additional gamma Tubulin Products

Product Documents for gamma Tubulin Antibody (9D6) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for gamma Tubulin Antibody (9D6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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