gamma Tubulin Antibody (TU-32) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-90616
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Porcine, Animal, Plant, Protozoa
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # TU-32
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449
Epitope
The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 29212260).
Specificity
The TU-32 recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
Marker
Centrosome Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for gamma Tubulin Antibody (TU-32) - BSA Free
Western Blot: gamma Tubulin Antibody (TU-32)BSA Free [NB110-90616]
Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Expression of the mitochondrial biogenesis proteins: SIRT1, PGC1alpha, TFAM and SIRT3 in H1299, H1299r, P31 and P31r cell lysates as determined by immunoblot analysis. Whole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 and P31r cells. Proteins (90 ug) were resolved in 10% SDS-PAGE gels and transferred to a PVDF membrane. Blots were probed for SIRT1, PGC1alpha, TFAM and SIRT3 or the loading control gamma-tubulin. Figure shows representative blots of (A) of SIRT1, (B) PGC1alpha, (C) SIRT3, (D) TFAM protein expression; each from three independent experiments. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885) licensed under a CC-BY license.Western Blot: gamma Tubulin Antibody (TU-32)BSA Free [NB110-90616]
Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Analysis of gamma-Tubulin in porcine brain lysate using NBP1-90616.Western Blot: gamma Tubulin Antibody (TU-32)BSA Free [NB110-90616]
Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.Applications for gamma Tubulin Antibody (TU-32) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1 ug/ml
Application Notes
Immunocytochemistry: methanol/acetone fixation required
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: gamma Tubulin
Long Name
Tubulin gamma-1 chain
Alternate Names
Gamma-1-tubulin, GCP-1, TUBG, TUBG1
Gene Symbol
TUBG1
UniProt
Additional gamma Tubulin Products
Product Documents for gamma Tubulin Antibody (TU-32) - BSA Free
Product Specific Notices for gamma Tubulin Antibody (TU-32) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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