Gankyrin Antibody (OTI3F6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71267
![Western Blot: Gankyrin Antibody (OTI3F6)Azide and BSA Free [NBP2-71267]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-OTI3F6-Azide-and-BSA-Free-Western-Blot-NBP2-71267-img0001.jpg "Western Blot: Gankyrin Antibody (OTI3F6)Azide and BSA Free [NBP2-71267]")
![Western Blot: Gankyrin Antibody (OTI3F6)Azide and BSA Free [NBP2-71267]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-OTI3F6-Azide-and-BSA-Free-Western-Blot-NBP2-71267-img0002.jpg "Western Blot: Gankyrin Antibody (OTI3F6)Azide and BSA Free [NBP2-71267]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3F6
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PSMD10 (NP_002805) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Gankyrin Antibody (OTI3F6) - Azide and BSA Free
Western Blot: Gankyrin Antibody (OTI3F6)Azide and BSA Free [NBP2-71267]
Western Blot: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD10.![Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-OTI3F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71267-img0008.jpg "Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]")
Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]
Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267] - Staining of paraffin-embedded Kidney tissue using anti-PSMD10mouse monoclonal antibody.![Flow Cytometry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-OTI3F6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71267-img0004.jpg "Flow Cytometry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]")
Flow Cytometry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]
Flow Cytometry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267] - Analysis of Jurkat cells, using anti-PSMD10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).![Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-OTI3F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71267-img0005.jpg "Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]")
![Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-OTI3F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71267-img0006.jpg "Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]")
![Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-OTI3F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71267-img0007.jpg "Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]")
![Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-OTI3F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71267-img0009.jpg "Immunohistochemistry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]")
![Flow Cytometry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]](https://resources.bio-techne.com/images/products/Gankyrin-Antibody-OTI3F6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71267-img0003.jpg "Flow Cytometry: Gankyrin Antibody (OTI3F6) - Azide and BSA Free [NBP2-71267]")
Applications for Gankyrin Antibody (OTI3F6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Gankyrin
Alternate Names
26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, ankyrin repeat protein, dJ889N15.2, Gankyrin, hepatocellular carcinoma-associated protein p28-II, p28, p28(GANK), proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
Gene Symbol
PSMD10
Additional Gankyrin Products
Product Documents for Gankyrin Antibody (OTI3F6) - Azide and BSA Free
Product Specific Notices for Gankyrin Antibody (OTI3F6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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