Gankyrin Antibody

Western Blot: Gankyrin Antibody [NBP1-82443]

Western Blot: Gankyrin Antibody [NBP1-82443] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-PSMD10 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443]

Immunocytochemistry/Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Staining of human cell line A-431 shows positivity in cytoplasm.

Knockdown Validated: Gankyrin Antibody [NBP1-82443]

Knockdown Validated: Gankyrin Antibody [NBP1-82443] - Effects of Gankyrin knock-down on Gankyrin and POU5F1 expression. Representative Western blot for Gankyrin and POU5F1 expression in (V) vehicle and Gankyrin siRNA (T) transfected NTera2 cells. Tubulin was used as a loading control. Data analysed by paired t-test +/- SEM, ***p < 0.001, **p < 0.01. Each data point represents the mean of an individualexperiment, each with 3 replicates. Paired samples from an individual experiment are represented by the same colour. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-019-6340-7), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] -

Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Representative images for Gankyrin expression in human fetal testis & normal adult testis. Gankyrin (red), POU5F1 (green; gonocyte) & MAGE-A4 (blue; prespermatogonia) expression in A), 11 week & B) 18 week old human fetal testis, counterstained with DAPI, yellow arrows – gonocytes, white arrows – pre-spermatogonia, green arrows – differentiating gonocyte, orange arrows – Sertoli cells, Scalebars – 50 μm. C) Representative image of Gankyrin expression in normal human adult testis immunostained for SOX9 (cyan; Sertoli cells), POU5F1 (green), MAGE-A4 (blue; spermatogonia) & Gankyrin (red), Cyan arrow – spermatogonia. Scalebars – 50 μm, insets – no primary antibody controls. w-weeks. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] -

Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Gankyrin expression pre-invasive germ cells. a) 1 year old - maturation delay; b) 2 year old – maturation delay; c) 7 year old pre-invasive TGCC; d)17 year old pre-invasive TGCC; & e) 23 year old pre-invasive TGCC. Yellow arrows – GCNIS cells, orange arrows – Sertoli cells. This experiment was performed along with human fetal testis samples, no primary antibody control is the same as on Fig. 1, Scalebars - 50 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] -

Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Representative images for Gankyrin expression in human fetal testis & normal adult testis. Gankyrin (red), POU5F1 (green; gonocyte) & MAGE-A4 (blue; prespermatogonia) expression in A), 11 week & B) 18 week old human fetal testis, counterstained with DAPI, yellow arrows – gonocytes, white arrows – pre-spermatogonia, green arrows – differentiating gonocyte, orange arrows – Sertoli cells, Scalebars – 50 μm. C) Representative image of Gankyrin expression in normal human adult testis immunostained for SOX9 (cyan; Sertoli cells), POU5F1 (green), MAGE-A4 (blue; spermatogonia) & Gankyrin (red), Cyan arrow – spermatogonia. Scalebars – 50 μm, insets – no primary antibody controls. w-weeks. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] -

Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Representative images for Gankyrin expression in human fetal testis & normal adult testis. Gankyrin (red), POU5F1 (green; gonocyte) & MAGE-A4 (blue; prespermatogonia) expression in A), 11 week & B) 18 week old human fetal testis, counterstained with DAPI, yellow arrows – gonocytes, white arrows – pre-spermatogonia, green arrows – differentiating gonocyte, orange arrows – Sertoli cells, Scalebars – 50 μm. C) Representative image of Gankyrin expression in normal human adult testis immunostained for SOX9 (cyan; Sertoli cells), POU5F1 (green), MAGE-A4 (blue; spermatogonia) & Gankyrin (red), Cyan arrow – spermatogonia. Scalebars – 50 μm, insets – no primary antibody controls. w-weeks. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Gankyrin Antibody [NBP1-82443] -

Western Blot: Gankyrin Antibody [NBP1-82443] - Effects of Gankyrin knock-down on Gankyrin & POU5F1 expression. Relative Gankyrin mRNA(a) & protein (b) expression in NTera2 cells after Gankyrin knock-down. Relative POU5F1 mRNA (c) & protein (d) expression in NTera2 cells after Gankyrin knock-down. e Representative Western blot for Gankyrin & POU5F1 expression in (V) vehicle & Gankyrin siRNA (T) transfected NTera2 cells. Tubulin was used as a loading control. Data analysed by paired t-test ± SEM, ***p < 0.001, **p < 0.01. Each data point represents the mean of an individualexperiment, each with 3 replicates. Paired samples from an individual experiment are represented by the same colour. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] -

Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Representative images for Gankyrin expression in human fetal testis & normal adult testis. Gankyrin (red), POU5F1 (green; gonocyte) & MAGE-A4 (blue; prespermatogonia) expression in A), 11 week & B) 18 week old human fetal testis, counterstained with DAPI, yellow arrows – gonocytes, white arrows – pre-spermatogonia, green arrows – differentiating gonocyte, orange arrows – Sertoli cells, Scalebars – 50 μm. C) Representative image of Gankyrin expression in normal human adult testis immunostained for SOX9 (cyan; Sertoli cells), POU5F1 (green), MAGE-A4 (blue; spermatogonia) & Gankyrin (red), Cyan arrow – spermatogonia. Scalebars – 50 μm, insets – no primary antibody controls. w-weeks. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.