GAP-43 Antibody (8E7C8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15636
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8E7C8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 139-238 of human GAP-43 (P17677). ETESATKASTDNSPSSKAEDAPAKEEPKQADVPAAVTAAAATTPAAEDAAAKATAQPPTETGESSQAEENIEAVDETKPKESARQDEGKEEEPEADQEHA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GAP-43 Antibody (8E7C8)
![Western Blot: GAP-43 Antibody (8E7C8) [NBP3-15636]](https://resources.bio-techne.com/images/products/GAP-43-Antibody-8E7C8-Western-Blot-NBP3-15636-img0002.jpg "Western Blot: GAP-43 Antibody (8E7C8) [NBP3-15636]")
Western Blot: GAP-43 Antibody (8E7C8) [NBP3-15636]
Western Blot: GAP-43 Antibody (8E7C8) [NBP3-15636] - Western blot analysis of extracts of various cell lines, using GAP-43 antibody (NBP3-15636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunohistochemistry: GAP-43 Antibody (8E7C8) [NBP3-15636]](https://resources.bio-techne.com/images/products/GAP-43-Antibody-8E7C8-Immunohistochemistry-NBP3-15636-img0001.jpg "Immunohistochemistry: GAP-43 Antibody (8E7C8) [NBP3-15636]")
Immunohistochemistry: GAP-43 Antibody (8E7C8) [NBP3-15636]
Immunohistochemistry: GAP-43 Antibody (8E7C8) [NBP3-15636] - Immunofluorescence analysis of rat retina using GAP-43 Rabbit mAb (NBP3-15636) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for GAP-43 Antibody (8E7C8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GAP-43
Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.
Long Name
Growth Associated Protein 43
Alternate Names
B-50, Basp2, GAP43, Neuromodulin
Gene Symbol
GAP43
Additional GAP-43 Products
Product Documents for GAP-43 Antibody (8E7C8)
Product Specific Notices for GAP-43 Antibody (8E7C8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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