GAP-43 Antibody (CL10847) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44282

Novus Biologicals, part of Bio-Techne
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NBP3-44282
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BSA Free
NBP3-21181
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL10847

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all GAP-43 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P17677, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for GAP-43 Antibody (CL10847) - Azide and BSA Free

GAP-43 Antibody (CL10847) - Azide and BSA Free Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]

Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-44282 antibody. Corresponding GAP-43 RNA-seq data are presented for the same tissues.
GAP-43 Antibody (CL10847) - Azide and BSA Free Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]

Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]

Staining of human cerebellum shows moderate positivity in neuropil.
GAP-43 Antibody (CL10847) - Azide and BSA Free Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]

Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP3-44282]

Staining of human cerebral cortex shows strong positivity in neuronal processes in neuropil.
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Applications for GAP-43 Antibody (CL10847) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAP-43

GAP43 (Growth associated protein 43) is a crucial component for regenerative response in the nervous system. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The protein is present at high levels in neuronal growth cones during development and axonal regeneration.

Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.

Long Name

Growth Associated Protein 43

Alternate Names

B-50, Basp2, GAP43, Neuromodulin

Gene Symbol

GAP43

Additional GAP-43 Products

Product Documents for GAP-43 Antibody (CL10847) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAP-43 Antibody (CL10847) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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