GAP-43 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41123
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Feline, Hamster, Primate
Cited:
Human, Mouse, Rat, Hamster
Applications
Validated:
Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
GAP-43 from rat brain
Localization
Cell membrane; Peripheral membrane protein; Cytoplasmic side
Marker
Neuronal Marker
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GAP-43 Antibody
Immunohistochemistry: GAP-43 Antibody [NBP1-41123]
Immunohistochemistry: GAP-43 Antibody [NBP1-41123] - Coronal sections of the developing (A-H) eye at (A-D) E14.5, (E-H) E17.5. were immunostained for (A-H) EVA1C and (A-H) GAP43. All sections were counterstained with DAPI. EVA1C was strongly co-expressed with GAP43 by retinal cell ganglionic axons (A-H) as they exit the retina and form the optic nerve (arrowheads). Scale bars represent 100 uM. PLoS One. 2013 Sep 9;8(9):e74115. doi: 10.1371/journal.pone.0074115.Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NBP1-41123]
Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP1-41123] - Staining of a mature retinal ganglion cell demonstrating axon outgrowth.Western Blot: GAP-43 Antibody [NBP1-41123]
Western Blot: GAP-43 Antibody [NBP1-41123] - Longitudinal [11C]GV1-57 imaging monitors mature OSN regeneration. Immunoblotting of immature neurons (GAP-43), mature neurons (OMP), and sustentacular cells (REEP6) in septal OE tissue collected from animals 2 or 11 weeks after ZnSO4 or vehicle treatment. Red arrows indicate DVR values and the corresponding immunoblots of OE tissue collected from the same individual animals 11 weeks after treatment. Purple arrows indicate DVR values representative of [11C]GV1-57 imaging 2 weeks after treatment and corresponding immunoblots of OE tissue collected from a separate cohort of animals. n = 2 per group at 2 weeks after treatment; n = 1 per group at 11 weeks after treatment. Image collected and cropped by CiteAb from the following publication (https://www.jci.org/articles/view/89162), licensed under a CC-BY license.Applications for GAP-43 Antibody
Application
Recommended Usage
Immunoblotting
reported in scientific literature (PMID 28112682)
Immunocytochemistry/ Immunofluorescence
1:50-1:1000
Immunohistochemistry
1:400-1:1000
Immunohistochemistry-Frozen
1:400-1:1000
Immunohistochemistry-Paraffin
reported in scientific literature (PMID 24686445)
Western Blot
1:1000
Application Notes
In Western blot, a band can be seen at 43-48 kDa.
Reviewed Applications
Read 1 review rated 4 using NBP1-41123 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GAP-43
Long Name
Growth Associated Protein 43
Alternate Names
B-50, Basp2, GAP43, Neuromodulin
Entrez Gene IDs
14432 (Mouse)
Gene Symbol
GAP43
Additional GAP-43 Products
Product Documents for GAP-43 Antibody
Product Specific Notices for GAP-43 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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