GAPDH-2 Antibody (2E3-2E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00026330-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, E. coli

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E3-2E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GAPDH-2 Antibodies »

Product Specifications

Immunogen

GAPDS (AAH36373, 1 a.a. ~ 408 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK

Reactivity Notes

Human. Other species not tested.

Specificity

GAPDHS - glyceraldehyde-3-phosphate dehydrogenase, spermatogenic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for GAPDH-2 Antibody (2E3-2E10)

Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01]

Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01]

Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01]

Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01]

Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.
Sandwich ELISA: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.

Applications for GAPDH-2 Antibody (2E3-2E10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GAPDH-2

This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.

Long Name

Glyceraldehyde-3-phosphate Dehydrogenase 2

Alternate Names

GAPD2, GAPDH2, GAPDHS, HSD-35

Entrez Gene IDs

26330 (Human)

Gene Symbol

GAPDHS

OMIM

609169 (Human)

UniProt

AAH36373 (Human)

Additional GAPDH-2 Products

Product Documents for GAPDH-2 Antibody (2E3-2E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAPDH-2 Antibody (2E3-2E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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