GAPDH-2 Antibody (Hs-8)
Novus Biologicals, part of Bio-Techne | Catalog # NB500-456
Key Product Details
Species Reactivity
Human, Porcine
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # Hs-8
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This GAPDH-2 antibody was developed against the full length human protein extracted from sperms.
Specificity
The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, previously identified under the general name "intra-acrosomal protein".
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for GAPDH-2 Antibody (Hs-8)
Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456]
Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456] - Detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456]
Separation of human GAPDHS positive cells (red-filled) from GAPDHS negative cells (black-dashed) in Analysis (intracellular staining) of human sperm cells stained using anti-GAPDHS (Hs-8) purified antibody (concentration in sample 7.5 ug/ml) GAM APC.Applications for GAPDH-2 Antibody (Hs-8)
Application
Recommended Usage
Flow Cytometry
3-12 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Western Blot
1:1000
Application Notes
Western Blot Detects bands of approximately 220, 50, 34 and 21 kDa. Immunocytochemistry: Staining technique: Membrane permeabilization (acetone) is essential.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
TBS (pH 8.0)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: GAPDH-2
Long Name
Glyceraldehyde-3-phosphate Dehydrogenase 2
Alternate Names
GAPD2, GAPDH2, GAPDHS, HSD-35
Entrez Gene IDs
56 (Human)
Gene Symbol
GAPDHS
Additional GAPDH-2 Products
Product Documents for GAPDH-2 Antibody (Hs-8)
Product Specific Notices for GAPDH-2 Antibody (Hs-8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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