GAPDH Antibody (13H12) [Alexa Fluor® 594]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27103AF594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Drosophila, Monkey, Primate, Sheep
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 13H12
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Amino acids between 275 and 325 of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen for this GAPDH antibody.
Reactivity Notes
Based upon 91% sequence similarity with immunogen, this antibody is predicted to react with Guinea Pig, Sheep, Squirrel, Porcine/Pig, Ferret, Canine/Dog/Cat, Bovine, Reptile / Rattlesnake and several other species. Immunogen shows 82% similarity to Xenopus and Zebrafish. Rat, sheep, and monkey reactivity reported in scientific literature (PMID: 24796753, PMID: 27618403, and PMID: 24462973 respectively).
Localization
Cytoplasm, Nucleus
Marker
Cytosolic Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications for GAPDH Antibody (13H12) [Alexa Fluor® 594]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: GAPDH
References
1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908
2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079
3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169
4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139
5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968
Long Name
Glyceraldehyde-3-phosphate Dehydrogenase
Alternate Names
G3PDH
Gene Symbol
GAPDH
Additional GAPDH Products
Product Documents for GAPDH Antibody (13H12) [Alexa Fluor® 594]
Product Specific Notices for GAPDH Antibody (13H12) [Alexa Fluor® 594]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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