GAPDH Antibody

Immunohistochemistry: GAPDH Antibody [NB300-322]

Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB

Western Blot: GAPDH Antibody [NB300-322]

Western Blot: GAPDH Antibody [NB300-322] - c-Src is required for efficient translation of PRC2 component mRNAs. Immunoblot of PRC2 components in control and c-Src-deficient tumors. Bar chart shows quantification of fluorescent immunoblot data normalized to the loading control (Gapdh). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10681-4), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-322]

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-322] - GAPDH detection in HeLa cells with ICC-IF application using NB300-322, visualized with DyLight Fluor 488.

Immunohistochemistry: GAPDH Antibody [NB300-322]

Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB

Western Blot: GAPDH Antibody [NB300-322]

Western Blot: GAPDH Antibody [NB300-322] - Mouse DRG stained at 1:2000 dilution. Image provided by verified customer review.

Western Blot: GAPDH Antibody [NB300-322]

Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase chemokinesis and chemotaxis. Western blot analyses were performed on 70 ug of cellular extracts from MOLT4 cells transduced by Lenti-control or Lenti-GEM viral particles. Membranes were probed with anti-Gem (1:2,000) or anti-GAPDH (1:1,000) antibody. ***: significantly different, p<0.0001, Student's t-test. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1003917) licensed under a CC-BY license

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322]

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of human lung carcinoma using NB300-322 at a dilution of 1:200.

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322]

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of mouse squamous cell carcinoma using NB300-322 at a dilution of 1:200.

Simple Western: GAPDH Antibody [NB300-322] -

Time-dependent effects of subacute administration of effective dose of escitalopram (5 mg/kg/day) for 3 & 7 days on expression of 5-HT7R in the thalamic plasma membrane fraction (Panel (A)). Ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R in the thalamic plasma membrane fraction. Panel (B) indicates the pseudo-gel images using capillary immunoblotting. ** p < 0.01 vs. control by one-way analysis of variance with Tukey’s post-hoc test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33572981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Concentration-dependent effects of subchronic administration of ZTP on Cx43 protein expression in the plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Concentration-dependent effects of ZTP (100, 300 & 1000 nM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by one-way ANOVA with Tukey’s (wholly significant difference) post hoc test (** p < 0.01 vs. ZTP free (0)). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Concentration-dependent effects of subchronic administration of ZTP on Cx43 protein expression in the cytosol fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Concentration-dependent effects of ZTP (100, 300 & 1000 nM) on Cx43 expression in the cytosol fraction of the primary cultured astrocytes were analysed by one-way ANOVA with Tukey’s (wholly significant difference) post hoc test (* p < 0.05 vs. ZTP free (0)). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Effects of acute (120 min) administration of supratherapeutic concentration of ZTP (1000 nM) & chronic (14 days) administration of therapeutically relevant concentrations of ZTP (300 nM) on Cx43 protein expression in the plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of ZTP on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by student T-test (* p < 0.05 vs. control: ZTP free). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Interaction between subchronic administration of supratherapeutic concentration of ZTP & Akt inhibitor (DEBC) on Cx43 protein expression in the astroglial plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of ZTP (1000 nM) & Akt inhibitor (DEBC: 10 μM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by two-way ANOVA with Tukey’s (wholly significant difference) post hoc test (** p < 0.01 vs. control, @@ p < 0.01 vs. non). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Simple Western: GAPDH Antibody [NB300-322] -

Simple Western: GAPDH Antibody [NB300-322] - Effects of subchronic nicotine administration on the expression of phosphorylated protein kinase B (pAkt) & phosphorylated extracellular signal-regulated kinase (pErk) in the plasma membrane fraction of OFC. Effects of the systemic subchronic administration of nicotine (50 mg/kg/day for seven days) on pAkt & pErk expression in the OFC plasma membrane fraction before four week of age (A,C) & after 12 week of age (B,D), ADSHE onset of the wild-type & S286L-TG & pseudo-gel images, using capillary immunoblotting. Ordinate: mean ± SD (n = 6) of the relative protein level of pErk & pAkt. * p < 0.05, ** p < 0.01 vs. wild-type, & @ p < 0.05, @@ p < 0.01 vs. nicotine-free (non) by two-way ANOVA with Tukey’s multiple comparison. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33143372), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Simple Western: GAPDH Antibody [NB300-322] -

Simple Western: GAPDH Antibody [NB300-322] - Effects of the subacute administration of effective doses of vortioxetine (2.5 mg/kg/day), escitalopram (5 mg/kg/day), & lurasidone (3 mg/kg/day) for 3 days on the expression of 5-HT7R in the thalamic plasma membrane fraction (Panel (A)). Ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R in the thalamic plasma membrane fraction. Panel (B) indicates the pseudo-gel images using capillary immunoblotting. * p < 0.05, ** p < 0.01 vs. the control by Student’s t-test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33572981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase cell motility.(A): HeLa cells were transduced with Lenti-control or Lenti-Tax viral particles. When cells reached 100% confluence, a wound was made in the cell monolayer & pictures were taken at 0 h & up to 12 h post-wounding. Measurements between the 2 fronts of migration were performed using image-J software (NIH, USA). (B): Percentage of healing during a 12 h kinetic. (C): Western blot analyses were performed on 70 µg of cellular extracts transduced by Lenti-control or Lenti-Tax viral particles. Membranes were probed with anti-Gem (1∶2,000) or anti-GAPDH (1∶1,000) antibody. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase chemokinesis & chemotaxis.(A, C): MOLT4 cells were transduced with Lenti-control or Lenti-GEM. Forty-eight hours later, 5.105 cells were collected & loaded on a 5 µm permeable Transwell filter in absence or presence of SDF1/CXCL12 (150 ng/ml). Twenty-four hours later, cell migration was quantified by flow cytometry (flow cytometer Facscalibur4c+HTS (BD biosciences)). (B, D): Western blot analyses were performed on 70 µg of cellular extracts from MOLT4 cells transduced by Lenti-control or Lenti-GEM viral particles. Membranes were probed with anti-Gem (1∶2,000) or anti-GAPDH (1∶1,000) antibody. ***: significantly different, p<0.0001, Student's t-test. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Effects of subchronic administration of therapeutic-relevant concentration of antipsychotics, CLZ (3 μM), QTP (1 μM) & BPZ (0.3 μM), on Cx43 protein expression in the plasma fraction (A) & their pseudo-gel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34070699), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Interaction between acute administration of therapeutic-relevant concentration of antipsychotics & Akt inhibitor (DEBC) on Cx43 protein expression in the astroglial plasma membrane fraction, after subchronic administration of therapeutic-relevant concentration of VPA (500 μM) (A). Interaction between subchronic administration of therapeutic-relevant concentration of antipsychotics & DEBC on Cx43 protein expression in the astroglial plasma membrane fraction, after subchronic administration of therapeutic-relevant concentration of VPA (B). Lower panels indicate their pseudo-gel images, using capillary immunoblotting. Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of antipsychotics & Akt inhibitor (DEBC: 10 μM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analyzed by MANOVA with Tukey’s post hoc test (** p < 0.01 vs. non, @@ p < 0.01 vs. control). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34070699), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Gem is overexpressed in T- & non-T-Tax-expressing cells as well as in HTLV-1 infected cells.(A): Total RNA was extracted from 293T cells transduced with Lenti-control (lane 2) or Lenti-Tax (lane 3) & RT-PCR was performed using gem or GADPH specific primers. Lane 1 is a control of extraction. (B, C): Western blot analyses were performed with 70 µg of proteins from (B) 293T or (C) MOLT4 cells transduced by Lenti-control or Lenti-Tax vectors (72 h post-transduction). (D): JPX-9 cells were grown for 24 h or 48 h in the presence of ZnCl2 (120 µM). Western blot analyses were performed with 70 µg of JPX-9 cellular extracts. (E): Western blot analyses were performed with 70 µg of cellular extracts obtained from non-infected (CEM, Jurkat & MOLT4) or HTLV-1-infected (C8166, C91/PL, Hut102 & MT2) T-lymphocytes. (B, C, D, E): Membranes were probed with anti-Gem (1∶2,000) & anti-GAPDH (1∶1,000) or anti-Tax Tab 172 (1∶500) antibodies. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Gem is overexpressed in T- & non-T-Tax-expressing cells as well as in HTLV-1 infected cells.(A): Total RNA was extracted from 293T cells transduced with Lenti-control (lane 2) or Lenti-Tax (lane 3) & RT-PCR was performed using gem or GADPH specific primers. Lane 1 is a control of extraction. (B, C): Western blot analyses were performed with 70 µg of proteins from (B) 293T or (C) MOLT4 cells transduced by Lenti-control or Lenti-Tax vectors (72 h post-transduction). (D): JPX-9 cells were grown for 24 h or 48 h in the presence of ZnCl2 (120 µM). Western blot analyses were performed with 70 µg of JPX-9 cellular extracts. (E): Western blot analyses were performed with 70 µg of cellular extracts obtained from non-infected (CEM, Jurkat & MOLT4) or HTLV-1-infected (C8166, C91/PL, Hut102 & MT2) T-lymphocytes. (B, C, D, E): Membranes were probed with anti-Gem (1∶2,000) & anti-GAPDH (1∶1,000) or anti-Tax Tab 172 (1∶500) antibodies. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.