GAPDH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-324

Novus Biologicals, part of Bio-Techne
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NB300-324
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Xenopus

Cited:

Human, Mouse

Predicted:

Amphibian (100%), Avian (100%), Chicken (100%), Guinea Pig (100%), Porcine (100%), Primate (100%), Rat (100%), Reptile (100%), Turkey (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all GAPDH Antibodies »

Product Specifications

Immunogen

This GAPDH antibody was developed against an epitope between residues 250 and 300 of human GAPDH [accession number NP_002037.2]

Reactivity Notes

Human and mouse. Based upon 100% sequence identity, this antibody is predicted to react with Rat, Chicken, Turkey, Guinea pig, Pig, Rhesus Monkey, Gorilla, Chimpanzee, West Indian ocean coelacanth, Neobatrachia, Sauropsida, Common iguana, Black-banded sea krait, Domestic pigeon and Indian bull frog.

Localization

Cytoplasm (cytosol, perinuclear region, cytoskeleton), Membrane, Nucleus

Marker

Cytosolic Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-324]

Western Blot: GAPDH Antibody [NB300-324]

Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH by Western Blot. Theoretical molecular weight: 36 kDa. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-324 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-324]

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-324]

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-324] - GAPDH antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: GAPDH Antibody [NB300-324]

Immunohistochemistry: GAPDH Antibody [NB300-324]

Immunohistochemistry: GAPDH Antibody [NB300-324] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB100-79808). Detection: DAB
View 4 more images

Applications for GAPDH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry-Paraffin

1:100 - 1:500

Simple Western

1:25

Western Blot

1:2000-1:10000
Application Notes
This GAPDH antibody is useful for Immunohistochemistry-paraffin, Immunocytochemistry/Immunofluorescence and Western blot applications. In WB, a specific band is observed at approx. 36 kDa molecular weight position. In Immunohistochemistry, epitope retrieval with citrate buffer pH6.0 is recommended for formalin-fixed paraffin-embedded tissue sections.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GAPDH

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis, converting glyceraldehyde-3-phosphate into 1,3 diphosphoglycerate. This constitutively expressed, homotetramer protein can be found in the nucleus and cytoplasm, and the monomer has a theoretical molecular weight of 36 kDa. Known as a housekeeping gene, GAPDH routinely serves as a control for real-time PCR (RT-PCR) and as a loading control for Western Blots (1-3). In addition to its role in glycolysis, GAPDH has multiple cellular functions including membrane trafficking, transcription activation, apoptosis, DNA repair, and has been implicated in various pathologies such as cancer and neurodegenerative diseases including Alzheimer's and Huntington's (4,5).

References

1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908

2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079

3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169

4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139

5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968

Long Name

Glyceraldehyde-3-phosphate Dehydrogenase

Alternate Names

G3PDH

Entrez Gene IDs

2597 (Human); 14433 (Mouse)

Gene Symbol

GAPDH

UniProt

P04406 (Human)

Additional GAPDH Products

Product Documents for GAPDH Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GAPDH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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