GAPDH Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-327
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bacteria, Bovine, Chicken, Equine, Fungi, Invertebrate, Yeast
Cited:
Human, Mouse, Rat, Avian - Chicken, Bacteria, Bovine, Fungi, Invertebrate, Yeast
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
This GAPDH antibody was developed against full length recombinant human GAPDH
Reactivity Notes
Bacteria reactivity reported in scientific literature (PMID: 31413153). Fungi reactivity reported in scientific literature (PMID:31413153).
Marker
Cytosolic Marker
Clonality
Polyclonal
Host
Rabbit
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GAPDH Antibody
Western Blot: GAPDH Antibody [NB300-327]
Western Blot: GAPDH Antibody [NB300-327] - Representative immunoblot of KDR and FGFR CAM protein expression levels examined by Western blotting. GNS, graphene nanosheet; NG, graphite nanoparticle; ND, diamond nanoparticle; C60, fullerene C60; MWNT, multi-wall nanotube; KDR, vascular endothelial growth factor receptor; FGFR, fibroblast growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Image collected and cropped by CiteAb from the following publication (https://nanoscalereslett.springeropen.com/articles/10.1186/1556-276X-8-195), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-327]
Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-327] - Analysis of HeLa cells stained with rabbit pAb to GAPDH, dilution 1:2000 in red. Blue is Hoescht staining of nuclear DNA. The GAPDH antibody produces diffuse cytoplasmic staining of cells.Western Blot: GAPDH Antibody [NB300-327]
Western Blot: GAPDH Antibody [NB300-327] - Theoretical molecular weight: 36 kDa. Detection of GAPDH in mouse liver.Applications for GAPDH Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500-1:1000
Immunohistochemistry
1:10000
Simple Western
1:500
Western Blot
1:5000
Application Notes
This GAPDH antibody is useful Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is observed at approx. 36kDa, and on cells in tissue culture the antibody stains in a punctate cytoplasmic fashion.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 3 reviews rated 5 using NB300-327 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Supplied as serum
Preservative
0.035% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GAPDH
References
1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908
2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079
3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169
4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139
5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968
Long Name
Glyceraldehyde-3-phosphate Dehydrogenase
Alternate Names
G3PDH
Gene Symbol
GAPDH
UniProt
Additional GAPDH Products
Product Documents for GAPDH Antibody
Product Specific Notices for GAPDH Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...