GART Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87290

Novus Biologicals, part of Bio-Techne
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NBP1-87290
NBP1-87290-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GART Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

108 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GART Antibody

Western Blot: GART Antibody [NBP1-87290]

Western Blot: GART Antibody [NBP1-87290]

Western Blot: GART Antibody [NBP1-87290] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: GART Antibody [NBP1-87290]

Immunohistochemistry-Paraffin: GART Antibody [NBP1-87290]

Immunohistochemistry-Paraffin: GART Antibody [NBP1-87290] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Western Blot: GART Antibody [NBP1-87290]

Western Blot: GART Antibody [NBP1-87290]

Western Blot: GART Antibody [NBP1-87290] - Analysis in human cell line HELA.

Applications for GART Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.In Western blots, detects both 108 kDa full-length protein and 46 kDa isomer.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GART

GART is encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

AIRS, EC 6.3.3.1, EC 6.3.4.13, GARS, GARTF, MGC47764, PGFTEC 2.1.2.210PAIS, phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamidesynthetase, phosphoribosylaminoimidazole synthetase, trifunctional purine biosynthetic protein adenosine-3

Entrez Gene IDs

2618 (Human)

Gene Symbol

GART

UniProt

P22102 (Human)

Additional GART Products

Product Documents for GART Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GART Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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