GATA-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2443
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DAEAYRHSPVFQ corresponding to internal region according to NP_002040.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for GATA-1 Antibody
Western Blot: GATA-1 Antibody [NB100-2443]
Western Blot: GATA-1 Antibody [NB100-2443] - Staining of K562 nuclear cell lysate (A) and negative control Human Hippocampus (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.Immunocytochemistry/ Immunofluorescence: GATA-1 Antibody [NB100-2443]
Immunocytochemistry/Immunofluorescence: GATA-1 Antibody [NB100-2443] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Flow Cytometry: GATA-1 Antibody [NB100-2443]
Flow Cytometry: GATA-1 Antibody [NB100-2443] - Analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for GATA-1 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:16000
Western Blot
0.3 - 1 ug/mL
Application Notes
Western blot: Approx 50-55 kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8 kDa according to NP_002040.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GATA-1
Additional GATA-1 Products
Product Documents for GATA-1 Antibody
Product Specific Notices for GATA-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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