GATA-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84793

Novus Biologicals, part of Bio-Techne
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NBP1-84793
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GATA-1 Antibodies »

Product Summary for GATA-1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GATA-1 Antibody

Western Blot: GATA-1 Antibody [NBP1-84793]

Western Blot: GATA-1 Antibody [NBP1-84793]

Western Blot: GATA-1 Antibody [NBP1-84793] - Analysis in control (vector only transfected HEK293T lysate) and GATA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: GATA-1 Antibody [NBP1-84793]

Immunohistochemistry-Paraffin: GATA-1 Antibody [NBP1-84793]

Immunohistochemistry-Paraffin: GATA-1 Antibody [NBP1-84793] - Staining in human bone marrow and pancreas tissues using anti-GATA1 antibody. Corresponding GATA1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GATA-1 Antibody [NBP1-84793]

Immunohistochemistry-Paraffin: GATA-1 Antibody [NBP1-84793]

Immunohistochemistry-Paraffin: GATA-1 Antibody [NBP1-84793] - Staining of human bone marrow shows high expression.
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Applications for GATA-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GATA-1

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Alternate Names

GATA1

Gene Symbol

GATA1

Additional GATA-1 Products

Product Documents for GATA-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GATA-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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