GATA-3 [p Ser308] Antibody (1E5) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26423
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1E5
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human GATA-3 [p Ser308] [UniProt P23771]
Modification
p Ser 308
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GATA-3 [p Ser308] Antibody (1E5) - BSA Free
![GATA-3 [p Ser308] Antibody (1E5)](https://resources.bio-techne.com/images/products/nbp3-26423_rabbit-gata-3-p-ser-308-mab-1e5-2722024856310.jpg "Immunohistochemistry: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] -")
Immunohistochemistry: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] -
Immunohistochemistry: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] - Image of GATA-3 [p Ser308] Antibody (1E5) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.![GATA-3 [p Ser308] Antibody (1E5)](https://resources.bio-techne.com/images/products/nbp3-26423_rabbit-gata-3-p-ser-308-mab-1e5-28220241135162.jpg "Western Blot: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] -")
Western Blot: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] -
Western Blot: GATA-3 [p Ser308] Antibody (1E5) [NBP3-26423] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate.All lanes: GATA-3 [p Ser308] Antibody at 2.5ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 48 KDa
Observed band size: 48 KDa
Applications for GATA-3 [p Ser308] Antibody (1E5) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: GATA-3
Alternate Names
GATA3
Gene Symbol
GATA3
Additional GATA-3 Products
Product Documents for GATA-3 [p Ser308] Antibody (1E5) - BSA Free
Product Specific Notices for GATA-3 [p Ser308] Antibody (1E5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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