GATA-3 Antibody (GATA3/2442)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07661
![Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]](https://resources.bio-techne.com/images/products/GATA-3-Antibody-GATA3-2442-Immunohistochemistry-Paraffin-NBP3-07661-img0002.jpg "Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # GATA3/2442
Concentration
0.2 mg/ml
Product Summary for GATA-3 Antibody (GATA3/2442)
Immunogen
Recombinant fragment of human GATA-3 protein (around aa 357-436) (exact sequence is proprietary) (Uniprot: P23771)
Localization
Nuclear
Specificity
GATA-3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA-3 expression is primarily seen in breast and urothelial carcinomas.'Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.
Marker
Breast and Urothelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08401)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for GATA-3 Antibody (GATA3/2442)
Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]
Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/2442).![Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]](https://resources.bio-techne.com/images/products/GATA-3-Antibody-GATA3-2442-Immunohistochemistry-Paraffin-NBP3-07661-img0001.jpg "Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]")
Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]
Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/2442).Applications for GATA-3 Antibody (GATA3/2442)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: GATA-3
Additional GATA-3 Products
Product Documents for GATA-3 Antibody (GATA3/2442)
Product Specific Notices for GATA-3 Antibody (GATA3/2442)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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