GATA-3 Antibody (GATA3/2442)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07661

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # GATA3/2442

Concentration

0.2 mg/ml

View all GATA-3 Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human GATA-3 protein (around aa 357-436) (exact sequence is proprietary) (Uniprot: P23771)

Localization

Nuclear

Specificity

GATA-3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA-3 expression is primarily seen in breast and urothelial carcinomas.'Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.

Marker

Breast and Urothelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08401)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for GATA-3 Antibody (GATA3/2442)

Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]

Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]

Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/2442).
Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]

Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]

Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/2442).

Applications for GATA-3 Antibody (GATA3/2442)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: GATA-3

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Alternate Names

GATA3

Gene Symbol

GATA3

UniProt

P23771

Additional GATA-3 Products

Product Documents for GATA-3 Antibody (GATA3/2442)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GATA-3 Antibody (GATA3/2442)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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