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GATA-3 Antibody (GATA3/2445)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07663

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-07663-100ug
NBP3-07663-20ug

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # GATA3/2445

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment of human GATA-3 protein (around aa 357-436) (exact sequence is proprietary) (Uniprot: P23771)

Localization

Nuclear

Specificity

GATA-3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA-3 expression is primarily seen in breast and urothelial carcinomas.'Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.

Marker

Breast and Urothelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08425)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for GATA-3 Antibody (GATA3/2445)

Protein Array: GATA-3 Antibody (GATA3/2445) [NBP3-07663]

Protein Array: GATA-3 Antibody (GATA3/2445) [NBP3-07663]

Protein Array: GATA-3 Antibody (GATA3/2445) [NBP3-07663] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GATA-3 Mouse Monoclonal Antibody (GATA3/2445).

Applications for GATA-3 Antibody (GATA3/2445)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: GATA-3

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Alternate Names

GATA3

Gene Symbol

GATA3

UniProt

Additional GATA-3 Products

Product Documents for GATA-3 Antibody (GATA3/2445)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GATA-3 Antibody (GATA3/2445)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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