GATA-3 Antibody (rGATA3/3870)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20264
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rGATA3/3870
Concentration
0.2 mg/ml
Product Summary for GATA-3 Antibody (rGATA3/3870)
Immunogen
Recombinant full-length human GATA-3 protein
Localization
Nucleus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Positive Controls: Human breast or urothelial carcinoma tissue.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.
Scientific Data Images for GATA-3 Antibody (rGATA3/3870)
GATA-3 Antibody (rGATA3/3870)
Western blot analysis of MCF-7 cell lysate using GATA-3 antibody (rGATA3/3870). Specific bands were detected for GATA-3 full length (FL) at approx. 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated).
GATA-3 Antibody (rGATA3/3870)
Formalin-fixed, paraffin-embedded human lobular breast carcinoma stained with GATA-3 antibody (rGATA3/3870). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
GATA-3 Antibody (rGATA3/3870)
Formalin-fixed, paraffin-embedded human urothelial carcinoma stained with GATA-3 antibody (rGATA3/3870).Applications for GATA-3 Antibody (rGATA3/3870)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: GATA-3
Alternate Names
GATA3
Gene Symbol
GATA3
Additional GATA-3 Products
Product Documents for GATA-3 Antibody (rGATA3/3870)
Product Specific Notices for GATA-3 Antibody (rGATA3/3870)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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