GATA-3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51912

Novus Biologicals, part of Bio-Techne
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NBP1-51912
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all GATA-3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence EVTADQPRWVSHH-C corresponding to N-Terminus according to NP_001002295.1, NP_002042.1.

Epitope

EVTADQPRWVSHH-C

Specificity

This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GATA-3 Antibody

Western Blot: GATA-3 Antibody [NBP1-51912]

Western Blot: GATA-3 Antibody [NBP1-51912]

Western Blot: GATA-3 Antibody [NBP1-51912] - Ataining of Human Tonsil (A) and Mouse Kidney (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for GATA-3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 47 kDa band observed in human tonsil, thymus, and human breast cancer lysates (calculated MW of 48 kDa band according to NP_001002295.1 and of 47.9 kDa band according to NP_002042.1). An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide. have any such results been reported with other antibodies/lysates?

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GATA-3

GATA3 is a 45-50 kD transcription factor found in the nucleus of T cells, mast cells, and embryonic brain and kidney that contains a GATA-type zinc finger motif. This transcription factor recognizes and binds the GATA core nucleotide motif (A/T) GATA (A/G) and has been shown to regulate T-H1 and T-H2 cell differentiation by directly binding to the IL-5 and IL-13 promoters, remodeling chromatin structure to open the IL-4 locus, and repressing IL-12R beta-2 chain expression. GATA3 has been shown to be induced by IL-4 through Stat6 and can be upregulated by positive feedback. GATA-3 can be modified by acetylation and has been shown to interact with Smad3. The Poly6071 antibody has been shown to be useful for Western blotting of human GATA3. Smad3

Alternate Names

GATA3

Entrez Gene IDs

2625 (Human); 14462 (Mouse)

Gene Symbol

GATA3

UniProt

NP_001002295.1 (Human)

Additional GATA-3 Products

Product Documents for GATA-3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GATA-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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