GATA-3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77115

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all GATA-3 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GATA3. The immunogen is located within amino acids 340 - 390 of GATA3. Amino Acid Squence: KEGIQTRNRKMSSKSKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GATA-3 Antibody - BSA Free

Western Blot: GATA-3 AntibodyBSA Free [NBP1-77115]

Western Blot: GATA-3 AntibodyBSA Free [NBP1-77115]

Western Blot: GATA-3 Antibody [NBP1-77115] - Analysis in K562 cell lysate with antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody - BSA Free [NBP1-77115]

Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody - BSA Free [NBP1-77115]

Immunocytochemistry/Immunofluorescence: GATA-3 Antibody [NBP1-77115] - Mouse brain tissue 20 ug/ml
Immunohistochemistry-Paraffin: GATA-3 Antibody - BSA Free [NBP1-77115]

Immunohistochemistry-Paraffin: GATA-3 Antibody - BSA Free [NBP1-77115]

Immunohistochemistry-Paraffin: GATA-3 Antibody [NBP1-77115] - Staining of mouse brain tissue with antibody at 2.5 ug/ml.
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Applications for GATA-3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GATA-3

GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer.

Alternate Names

GATA3

Gene Symbol

GATA3

UniProt

NP_001002295 (Human)

Additional GATA-3 Products

Product Documents for GATA-3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GATA-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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